The in vivo two-photon laser microscope has multiple core technical advantages. First, the 800nm-1040nm tunable femtosecond laser can match the excitation wavelengths of most common fluorescent dyes, enabling specific labeling and imaging of different biological structures without cross-interference. The laser energy is concentrated in a tiny focal volume, reducing photobleaching and photodamage to living samples, allowing long-term real-time observation of living biological processes. The gallium arsenide photomultiplier tube detector has high sensitivity to weak fluorescence signals, improving the signal-to-noise ratio of imaging results. The scan speed of 30fps at 512*512 resolution supports real-time capture of dynamic biological processes such as neuron firing and cell migration. The motorized XYZ scanning stage enables automatic sample scanning and 3D reconstruction, while the water-cooled cooling system ensures stable long-term operation of high-power lasers. The professional 3D live imaging analysis software supports functions such as calcium signal quantification, neuron trajectory tracking, and 3D volume rendering, greatly reducing the workload of researchers in post-processing analysis.
Technical parameters of this in vivo two-photon laser microscope are as follows: The laser source is an 800nm-1040nm tunable femtosecond laser, with a maximum output power of 3W. The magnification range is 10x to 60x, with a lateral imaging resolution of 0.3μm and an imaging depth of up to 1mm in biological tissue. The detector adopts gallium arsenide photomultiplier tubes, with a quantum efficiency of up to 45% at 500nm wavelength. The scan speed is 30fps at 512*512 resolution, with a maximum scan resolution of 2048*2048. The motorized XYZ scanning stage has a travel range of 100*100*50mm, with a positioning accuracy of ±0.1μm. The device is equipped with professional 3D live imaging analysis software, supporting calcium signal analysis, neuron tracking, 3D reconstruction, and other functions. The cooling system adopts water-cooled design, with a cooling power of 500W. The power supply is 220V 50/60Hz, with a rated power of 1.5kW. The whole machine has dimensions of 120*80*150cm and a net weight of 320kg, and is equipped with a 4TB external RAID storage array for large-capacity data storage. The laser safety class is Class 4, requiring dedicated protective equipment and a special operating room for safe use.
This in vivo two-photon laser microscope is widely used in cutting-edge life science research fields. In neuroscience, it can realize real-time imaging of living mouse brain neuron activity, capturing the firing process of single neurons and the interaction between neural circuits, providing key data for the study of brain function and neurological diseases. In cell biology, it can observe the dynamic processes of living cell proliferation, migration, and apoptosis, supporting research on cell signal transduction and drug action mechanisms. In pharmacological research, it can realize real-time monitoring of drug efficacy in living organisms, such as the distribution and metabolic process of anti-tumor drugs in tumor tissues. In plant biology, it can observe the microscopic structure of living plant tissues, such as the flow of cell sap and the growth of plant roots. Additionally, it can also be used in the fields of developmental biology and immunology, providing a powerful tool for in vivo real-time observation of biological processes.
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